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The Department is located on the second floor of the main building
at the Rizzi Campus and half of its overall area of 2000 sq. m.
is occupied by labs. Other facilities like stores, growth chambers
and refrigerated rooms are housed in the basement of the same
building.
The
University Computer Center is nearby on the same floor. The University
Experimental Station is in the outskirt of Udine, 5 km from the
Rizzi campus, and can be easily reached by bus, car or bicycle.
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Each
Section has its own labs, but co-operation between sections is very common
and exchanges at any level are encouraged. In the following description
labs are grouped and listed according to their destination.
Routine chemical analysis
Analysis carried out routinely on seed and/or fruit are: protein,
sugar, acids, amino-acids, lipids, plant hormones, chemical elements
like C, H, N, S. The basic instrumentation includes: HPLCs and GCs,
UV and visible spectrometers, Atomic adsorption spectrometer, 15N emission
spectrometer, elemental analyzer, ICP.
Plant physiology & biochemistry
Routine analysis are performed in vivo and in vitro. On intact plants
(cucumber, maize, oat, kiwifruit etc.) uptake and efflux of ions and
nutrients like H+, NO3-, K+ and enzymatic activities such as FeIII-reductase,
and chlorophyll content are measured . Plasma membrane-enriched fractions
from root tissues are isolated using methods of differential centrifugation
and sucrose gradients or two phase partitioning. Proteic content and
enzymatic activities such as H+-ATPase, FeIII-reductase and PEP carboxylase
are determined and measured on such fractions. Enzymatic activities
involved in peach fruit metabolism are also studied. The basic instrumentation
includes: Ultracentrifuge, EF apparatus, densitometer, flame spectrophotometer
and UV-vis spectrophotometer.
Soil chemistry & biochemistry
The routine analyses performed in these labs include: Humification index,
total organic carbon, soil clay content, biomass carbon, nitrogen, ninhydrin-N,
P, soil ATP and soil CO2 evolution and N mineralization, substrate induced
respiration. Extraction of different micronutrients and heavy metals
and extraction of humic substances are also performed. Separation and
chromatographic techniques like ultrafiltration and electrofocusing
are employed normally in our labs. The instruments of these labs are:
gas chromatographs, HPLC, organic carbon analyser, densitometer, flame
spectrophotometer, IR spectrophotometer, ultracentifuge, electrofocusing
apparatus, atomic absorption spectrophotometer and ß-counter.
Molecular biology
Molecular biology labs are physically separated in three areas dedicated
to: (a) genome analysis, (b) gene cloning and expression, and (c) transformation.
Genome analysis. In this area, molecular markers are isolated and assayed
for genetic analysis, genome analysis and mapping, population genetics,
fingerprinting. The basic instrumentation includes: PCRs, agarose and
acrilammide EF apparatus, A.L.F. DNA automatic sequencer, hybridisation
ovens, laminar-flow cabinets and Incubators for cloning. A room devoted
to procedures requiring the use of radionucletides (labelled-P) equipped
with PCR, sequencing gel EF apparatus and other is also available. Gene
cloning and gene expression. Labs include facilities required for RNA
and cDNA analysis: PCRs devoted to RNA, hybridisation oven, EF apparatus
for both DNA/RNA and proteins. Transformation. Transformations via Agrobacterium
and particle bombardment are currently carried out. Equipment includes:
laminar-flow cabinets, particle gun, incubators and growth chambers,
PCR and EF apparatus for both DNA and proteins.
Crop physiology & Ecology
The routine analyses performed in these labs are: sap flow and other
measurements of plant water relations, non-destructive leaf chlorophyll
content and gas-exchange, acetylene reduction as a tool to measure nodule
nitrogen fixation, elemental C-N-S analyses in plant tissues, growth
analysis of field crops, technological aspects of wheat flour quality,
fatty acids composition in oilseed crops. In these labs the following
instruments and equipments are used (some are also available for open
field activity): sensors for the main meteorological and micro-meteorological
parameters, laboratory and portable IRGA systems, chlorophyll meters,
sap flow gauges, mass flow meters, dew point meters, NMR analyzer, dataloggers,
growth bench, landfill gas analyzer, gel and capillary electrophoresis
systems, GC and HPLC systems, Elemental Analyzer CHN.
GIS (Geographical Information System)
The topics of research in the GIS laboratory include: monitoring changes
in alpine rangelands using aerial photographs and GIS; land use mapping
with remote sensing; evaluation of the application of agro-environmental
policies (Reg. 2078/92) in the Friuli-Venezia Giulia region; GIS applications
for landscape analysis, particularily landscape change, landscape mapping,
landscape assessment and landscape characterisation. The GIS Laboratory
is part of CARTESIO, the interdepartmental centre of the University
of Udine for research, education and training in Cartography, Remote
Sensing and Geographical Information Systems (GIS).The main facilities
of the laboratory are: Sun Sparc Station 10, 32 Mb RAM, 1Gb HD + 2 external
Gb; PC Pentium, 64 Mb RAM, 4 Gb HD; PC Pentium, 32 Mb RAM, 4 Gb HD,
PC Pentium, 32 Mb RAM, 2 Gb HD, Calcomp 9100 Digitizing table, Calcomp
A3 digitizing tablette, A4 scanner, HP Paintjet XL300 printer, Calcomp
1023 pen plotter, CD-ROM writer, MO drives, Jaz and Zip drives. The
GIS Lab has at its disposal some of the latest GIS software, including
ESRI ArcView 3.1, MapInfo 3.0, IDRISI 2.0 and Grass4.1
Experimental farm
The "A. Servadei" experimental
farm is located Udine South and is easily reached in 5' by car or
20' by bus or cycle.
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